Myeloid related protein induces muscle derived inflammatory mediators in juvenile dermatomyositis

Abstract : Introduction: The aetiopathogenesis of juvenile dermatomyositis (JDM) remains poorly understood. In particular the contribution of monocytes or macrophages, which are frequently observed to be an infiltrate within muscle tissue very early in the disease process, is unknown. We hypothesised that these cells secrete the pro-inflammatory myeloid related protein (MRP) 8/14 which may then contribute to muscle pathology in JDM. Methods: In this study of 56 JDM patients, serum MRP8/14 levels were compared with clinical measures of disease activity. Muscle biopsies taken early in disease were assessed by immunohistochemistry to determine the frequency and identity of MRP-expressing cells. The effects of MRP stimulation and endoplasmic reticulum (ER) stress on muscle were tested in vitro. Serum or supernatant levels of cytokines were analyzed by multiplex immunoassay. Results: Serum MRP8/14 correlated with physician’s global assessment of disease activity in JDM (R = 0.65, p = 0.0003) and muscle strength/endurance, childhood myositis assessment score (CMAS, R = −0.55, p = 0.004). MRP8/14 was widely expressed by CD68+ macrophages in JDM muscle tissue. When cultured with human myoblasts, MRP8 led to the secretion of MCP-1 and IL-6, which was enhanced by ER stress. Both inflammatory mediators were detected in significantly higher levels in the serum of JDM patients compared to healthy controls. Conclusions: This study is the first to identify serum MRP8/14 as a potential biomarker for disease activity in JDM. We propose that tissue infiltrating macrophages secreting MRP8/14 may contribute to myositis, by driving the local production of cytokines directly from muscle.
Type de document :
Article dans une revue
Arthritis Research and Therapy, BioMed Central, 2013, 15 (5), pp.R131. 〈10.1186/ar4311〉
Liste complète des métadonnées

Littérature citée [60 références]  Voir  Masquer  Télécharger

http://hal.upmc.fr/hal-01543043
Contributeur : Gestionnaire Hal-Upmc <>
Soumis le : mardi 20 juin 2017 - 14:17:16
Dernière modification le : mercredi 21 mars 2018 - 18:57:35
Document(s) archivé(s) le : vendredi 15 décembre 2017 - 19:24:24

Fichier

myeloid.pdf
Publication financée par une institution

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Collections

Citation

Kiran Nistala, Hemlata Varsani, Helmut Wittkowski, Thomas Vogl, Petra Krol, et al.. Myeloid related protein induces muscle derived inflammatory mediators in juvenile dermatomyositis. Arthritis Research and Therapy, BioMed Central, 2013, 15 (5), pp.R131. 〈10.1186/ar4311〉. 〈hal-01543043〉

Partager

Métriques

Consultations de la notice

143

Téléchargements de fichiers

73