Amorphous Surface Layer versus Transient Amorphous Precursor Phase in Bone - A Case Study Investigated by Solid-state NMR Spectroscopy

Stanislas Von Euw 1 Widad Ajili 1 Tsou-Hsi-Camille Chan-Chang 2 Annette Delices 1 Guillaume Laurent 1 Florence Babonneau 1 Nadine Nassif 3, 2 Thierry Azaïs 1, *
* Auteur correspondant
1 SMiLES - Spectroscopie, Modélisation, Interfaces pour L'Environnement et la Santé
LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris
2 MHN - Matériaux Hybrides et Nanomatériaux
LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris
3 MATBIO - Matériaux et Biologie
LCMCP - Laboratoire de Chimie de la Matière Condensée de Paris
Abstract : The presence of an amorphous surface layer that coats a crystalline core has been evidenced for many biominerals, including bone mineral. In parallel, transient amorphous precursor phases have been proposed in various biomineralization processes, including bone biomineralization. Here we propose a methodology to investigate the origin of these amorphous environments taking the bone tissue as a key example. This study relies on the investigation of a bone tissue sample and its comparison with synthetic calcium phosphate samples, including a stoichiometric apatite, an amorphous calcium phosphate sample, and two different biomimetic apatites. To reveal if the amorphous environments in bone originate from an amorphous surface layer or a transient amorphous precursor phase, a combined solid-state nuclear magnetic resonance (NMR) experiment has been used. The latter consists of a double cross polarization 1H→31P→1H pulse sequence followed by a 1H magnetization exchange pulse sequence. The presence of an amorphous surface layer has been investigated through the study of the biomimetic apatites; while the presence of a transient amorphous precursor phase in the form of amorphous calcium phosphate particles has been mimicked with the help of a physical mixture of stoichiometric apatite and amorphous calcium phosphate. The NMR results show that the amorphous and the crystalline environments detected in our bone tissue sample belong to the same particle. The presence of an amorphous surface layer that coats the apatitic core of bone apatite particles has been unambiguously confirmed, and it is certain that this amorphous surface layer has strong implication on bone tissue biogenesis and regeneration.
Type de document :
Article dans une revue
Acta Biomaterialia, Elsevier, 2017, 59, pp.351-360. 〈10.1016/j.actbio.2017.06.040〉
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Soumis le : mardi 11 juillet 2017 - 18:01:33
Dernière modification le : mercredi 21 mars 2018 - 18:58:22

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Stanislas Von Euw, Widad Ajili, Tsou-Hsi-Camille Chan-Chang, Annette Delices, Guillaume Laurent, et al.. Amorphous Surface Layer versus Transient Amorphous Precursor Phase in Bone - A Case Study Investigated by Solid-state NMR Spectroscopy. Acta Biomaterialia, Elsevier, 2017, 59, pp.351-360. 〈10.1016/j.actbio.2017.06.040〉. 〈hal-01559945〉

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