Both genome and cytosol dynamics change in E. coli challenged with sublethal rifampicin.

Abstract : While the action of many antimicrobial drugs is well understood at the molecular level, a systems-level physiological response to antibiotics remains largely unexplored. This work considers fluctuation dynamics of both the chromosome and cytosol in Escherichia coli, and their response to sublethal treatments of a clinically important antibiotic, rifampicin. We precisely quantify the changes in dynamics of chromosomal loci and cytosolic aggregates (a rheovirus nonstructural protein known as μNS-GFP), measuring short time-scale displacements across several hours of drug exposure. To achieve this we develop an empirical method correcting for photo-bleaching and loci size effects. This procedure allows us to characterize the dynamic response to rifampicin in different growth conditions, including a customised microfluidic device. We find that sub-lethal doses of rifampicin cause a small but consistent increase in motility of both the chromosomal loci and cytosolic aggregates. Chromosomal and cytosolic responses are consistent with each other and between different growth conditions.
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Article dans une revue
Physical Biology, Institute of Physics: Hybrid Open Access, 2017, 14 (1), pp.015005
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http://hal.upmc.fr/hal-01587958
Contributeur : Francesco Oteri <>
Soumis le : jeudi 14 septembre 2017 - 19:30:35
Dernière modification le : jeudi 11 janvier 2018 - 06:25:46

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  • HAL Id : hal-01587958, version 1
  • PUBMED : 28207419

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Michal Wlodarski, Bianca Raciti, Jurij Kotar, Marco Cosentino Lagomarsino, Gillian M Fraser, et al.. Both genome and cytosol dynamics change in E. coli challenged with sublethal rifampicin.. Physical Biology, Institute of Physics: Hybrid Open Access, 2017, 14 (1), pp.015005. 〈hal-01587958〉

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